Bacillus Medium – HX-MEDIA

Name: Bacillus Medium - HX-MEDIA
SKU: HXM-BM-01
Availability: InStock

Principle :- Bacillus medium is used for cultivation of Bacillus licheniformis, it is a gram-positive, spore-forming soil bacterium that is used in the biotechnology industry to manufacture enzymes, antibiotics, biochemicals and consumer products. This medium is modification of Thorne Medium (1966, 1992), recommended for cultivation of Bacillus licheniformis. Bacillus medium contains L-glutamic acid, citric acid, Dipotassium phosphate, ferric ammonium citrate and magnesium Sulphate. At time of preparation the media is fortified with glycerol, which provides carbon and energy for the growth of bacteria. Glutamic acid and ferric ammonium citrate act as source of nitrogen. Citric acid provides necessary nutrients along buffering action. Dipotassium phosphate acts as buffering agent. Magnesium Sulphate is necessary for the growth of microorganisms and provides membrane stability and act as cofactor in various metabolic reactions.

Use :- Recommended for cultivation of Bacillus licheniformis.

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Bacillus Medium – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 7.50 grams in 1000 ml distilled water containing 20.0 g of glycerol. Boil to dissolve medium completely. Bacillus Medium sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.

Specimens’ types analyzed :- Pharmaceutical and economically important isolates etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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