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Principle :- Koser citrate medium is described by Koser (1923), media composed of sodium ammonium phosphate, monobasic potassium phosphate, magnesium sulfate, sodium citrate. This media lacks organic carbon and nitrogen source. Sodium ammonium phosphate and sodium citrate are source of carbon and nitrogen. Monobasic potassium phosphate and sodium ammonium phosphate act as buffering agent. Magnesium sulfate is source of ions.
Use :- Recommended for differentiating Escherichia coli from Enterobacter aerogenes based on citrate utilization.
Directions :- Dissolve 5.7 grams in 1000 ml distilled water, boil Koser Citrate Medium to dissolve the medium completely and distribute aseptically in test tubes. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
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