Lauryl Sulphate Broth – HX-MEDIA

Name: Lauryl Sulphate Broth - HX-MEDIA
SKU: HXM-LSB-01
Availability: InStock

Principle :- Lauryl sulphate broth is composed of tryptose, lactose, potassium phosphate dibasic and monobasic, sodium chloride and sodium lauryl sulfate. Tryptose provides nitrogen and amino acids. Lactose is fermentable carbohydrate. Potassium phosphates are buffering agents. Sodium chloride maintain osmatic balance. Sodium lauryl sulfate inhibits non-coliforms. The selectivity to the medium is provide by lactose and sodium lauryl sulfate.

Use :- Recommended for detecting coliform organisms in water and wastewater.

Lauryl Sulphate Broth – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 35.60 grams in 1000 ml distilled water. Boil Lauryl Sulphate Broth to dissolve the medium completely and distribute in test tube containing inverted Durham’s tube. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
Note : for 1.0 or less than 1.0 ml of water sample single strength broth is used and for more than 1.0 ml double strength broth is used.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and
discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

-20%