Moeller Decarboxylase Broth Base – HX-MEDIA

Name: Moeller Decarboxylase Broth Base - HX-MEDIA
SKU: HXM-DCX-01
Availability: InStock

Principle :- Moeller Decarboxylase Broth Base is used for differentiation of enteric bacilli on basis of their ability to decarboxylate amino acids. The media is composed of peptone, special peptone, dextrose, bromocresol purple, cresol purple and pyridoxal. Peptone and special peptone provides nitrogen, vitamins, minerals, amino acids and growth factors. Dextrose is carbon or energy source for the growth of microorganisms. Pyridoxal is the co-factor for the decarboxylase enzyme. Bromo cresol purple and cresol red are the pH indicators. When dextrose is fermented by bacteria, the pH is lowered due to acid production, which changes the colour of the indicator from purple to yellow. Acid produced stimulates decarboxylase enzyme.

Use :- Recommended to differentiate bacteria on the basis of their ability to decarboxylate the amino acids.

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Moeller Decarboxylase Broth Base – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 10.50 grams in 1000 ml distilled water. Add Moeller Decarboxylase Broth Base 10 gm. of L-Lysine, L-Arginine, L-Ornithine or other L-amino acids. When using DL-amino acids, use 2% concentration (When L-Ornithine is added, adjust the pH to 6.0). Heat if necessary, to dissolve the medium completely. Distribute 5.0 ml in screw-capped tubes and sterilize by autoclaving at 15 lbs pressure (121 °C) for 10 min, Cool to 20-25°C and aseptically inoculate test sample.

Specimens’ types analyzed :- Clinical samples – – Blood; Food and dairy samples; Water samples

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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