Xylose Lysine Deoxycholate Agar – HX-MEDIA

Name: Xylose Lysine Deoxycholate Agar - HX-MEDIA
SKU: HXM-XLDA-01
Availability: InStock

Principle :- Xylose Lysine Deoxycholate Agar is a differential medium, slightly selective for the detection of pathogenic enterobacteria, especially Salmonella and Shigella. Media is composed of xylose, lysine, lactose monohydrate, sucrose, sodium chloride, yeast extract, phenol red, sodium deoxycholate, sodium thiosulphate, ferric ammonium citrate and agar. Yeast extract provides sources of nitrogen and carbon, as well as vitamins and cofactors required for growth. Xylose, lactose and sucrose are the carbon source. Xylose is fermented by most enteric organisms except Shigella and Providencia. Phenol red is pH indicator. The Salmonella colonies show red colored with black centers and Shigella are red colored without black center.

Use :- Recommended for the isolation and enumeration of Salmonella and Shigella from pharmaceutical products.

Xylose Lysine Deoxycholate Agar – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 55.20 grams in 1000 ml purified/ distilled water. Heat Xylose Lysine Deoxycholate Agar until the medium boils with frequent agitation. Do Not Autoclave or Overheat. Transfer immediately to a water bath at 50°C. After cooling, pour into sterile petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.

Specimens types analyzed :- Pharmaceutical samples, clinical and non-clinical samples etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving.

Performance and Evaluation :- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date. Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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