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Eosin Methylene Blue Broth
Eosin Methylene Blue Broth
HomeMicro BiologyAgar Media Eosin Methylene Blue Broth – HX-Media
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Eosin Methylene Blue Broth – HX-Media

₹595.00 – ₹4,595.00

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Eosin Methylene Blue Broth – HX-Media

Principle:- The Eosin Methylene Blue Broth is the combination of Levine and Holt-Harris and Teague formulae. The peptic digest of animal tissue serves as the source of Carbon, Nitrogen, and vitamins, while the two sugars and the dyes Methylene blue and Eosin Y inhibit the Gram positive bacteria. The EMB is a selective media, which differentiate between the lactose fermenters and non-lactose fermenters; some gram negative bacteria sometimes do not utilize the lactose or are very slow at lactose utilizing hence the sucrose is used as secondary carbohydrate source. The lactose non-fermenters such as Salmonella sp. raise the pH of surrounding by deamination of protein thus resulting in change of pH, solubilize the Eosin Methyl red complex and grow as colorless colonies. While the coliforms produce black purple colonies, such as Escherichia coli forms characteristic green colored metallic sheen. The major limitation of this medium is presence of Greenish metallic Sheen is not confirmatory for all strains of E coli as some strains may grow without developing the green metallic sheen.

Use:- Recommended for the differential isolation of the gram negative Enteric Bacilli from clinical and non-clinical samples.

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SKU: HXM-EMB-01 Categories: Agar Media, Micro Biology Tags: Broth, eosin methylene blue agar composition, eosin methylene blue agar himedia, eosin methylene blue agar results, Eosin Methylene Blue Broth, hexa biotech, HX-Media, ScienoChem
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Description

Eosin Methylene Blue Broth – HX-Media – Hexa Biotech

Directions:- Suspend 36.00 gram in 1000 ml distilled water adjust the pH if required. Boil to dissolve eosin methylene blue broth, the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically in to the plates.

Specimens types analyzed:- The Water samples, Soil samples, Food and dairy samples, Pharmaceutical samples, clinical samples, can directly streak plated.

Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life:- Hygroscopic; Keep container tightly closed. Store at below 30⁰C and dry place. Expected performance when stored at optimum conditions and within expiry date.

Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.

Standard Formula 
Ingredients Gram / Liter 
Peptone 10.000
Lactose 5.000
Sucrose 5.000
Eosin Y 0.400
Methylene Blue 0.065
Potassium Phosphate Dibasic 2.000
Agar 13.000
pH (at 25 ‘C) 7.2 ± 0.2
Additional information
Weight N/A
Dimensions N/A
Size

100G, 1Kg, 500G

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Eosin Methylene Blue Broth

Eosin Methylene Blue Broth – HX-Media

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