TAT Broth – HX-MEDIA

Name: TAT Broth - HX-MEDIA
SKU: HXM-TATB-01
Availability: InStock

Principle :- TAT Broth Base is an enrichment medium developed to isolate and cultivate microorganisms. TAT Broth Base conforms to the formula specified by US Pharmacopeia for use in Microbial Limit Tests. It is used for enrichment and further isolation and cultivation of gram-negative bacteria in cosmetics, tropical drugs and in the sterility testing of viscous or gelatinous substances. It consists of tryptone and azolectin. tryptone provides the nitrogen, vitamins, amino acids and carbon. Inoculate 1 gram (1 ml) sample and 1 ml of each dilution into 40 ml of TAT Broth and after incubation, subculture the growth on MacConkey Agar.

Use :- Recommended for enrichment and further isolation and cultivation of gram-negative bacteria in cosmetics, tropical drugs and in the sterility testing of viscous or gelatinous substance.

TAT Broth – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 25.00 grams in 1000 ml distilled water, add 40 ml of tween 20. Heat TAT Broth to 50-60°C for 15-30 minutes with occasional agitation till media dissolve completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.

Specimens types analyzed :- Ointments and other cosmetic products, viscous or gelatinous pharmaceutical samples, clinical and non-clinical samples etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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