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Murashige and Skoog Medium
HomeMicro BiologyAgar Media Murashige and Skoog Medium – HX-Media
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Murashige and Skoog Medium – HX-Media

₹875.88 – ₹3,828.83

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Murashige and Skoog Medium – HX-Media

Principle:- MS medium formulated by Murashige and Skoog in 1962 for the tobacco callus culture. It is the blend of inorganic salts (Macro and Micro nutrients), vitamins, amino acids carbohydrates and gelling agent. Macro nutrients serve the source of the Nitrogen, Calcium, Magnesium, Potassium which are essential for the plant growth and metabolism. The micro nutrients Boron, Cobalt, copper, Ferrous, Manganese, Molybdenum, Iodine and Zinc play a crucial role in the plant metabolism. The vitamins such as myo inositol, Nicotinic acid, Pyridoxine and Thiamine acts as the co factor in the universal plant metabolic pathways such as glycolysis and TCA cycle. The glycine serves the source of basic amino acid. The Sucrose serves helps to initiation, normalization and synchronizes the plant cell growth and energy source.

Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

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SKU: HXM-MS-01 Categories: Agar Media, Micro Biology Tags: hexa biotech, HX-Media, Murashige and Skoog Medium, murashige and skoog medium composition, murashige and skoog medium for sale, murashige and skoog medium is used for, murashige and skoog medium preparation, murashige and skoog medium slideshare, ScienoChem
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Description

Murashige and Skoog Medium – HX-Media – Hexa Biotech

Murashige and Skoog Medium Precautions:-

  • The acidic pH may loosen ability of gelling, and the basic pH may harden the agar hence maintaining the optimum pH is recommended for better outcomes.
  • Use of distilled/ deionized water is recommended as the tap water or the potable water may lead to precipitation of inorganic salts.

Storage and Shelf Life:- Hygroscopic; keep container tightly closed. Store at temperatures below 25⁰C and dry place.

Disposal:- To avoid the contamination or propagation of any hazardous microbes used, unusable or modified preparation of this product must be disposed after autoclaving or incineration after completion of task.

Contents
Ingredient                                                    mg/ L Ingredient                               mg/ L
Macro Nutrients Potassium Iodide                          0.830
Ammonium nitrate                                            1650.000 Zinc Sulphate hepta-hydrate      8.600
Calcium chloride                                                  332.200 Vitamins
Magnesium Sulphate                                          180.690 myo-Inositol                              100.000
Potassium nitrate                                               1900.000 Nicotinic acid (free acid)             0.500
Potassium phosphate monobasic                     170.000 Pyridoxine HCl                              0.500
Micro Nutrients Thiamine hydrochloride             0.100
Boric acid                                                                  6.200 Amino Acid
Cobalt chloride hexa-hydrate                               0.025 Glycine                                           2.000
Copper Sulphate penta-hydrate                           0.025 Carbohydrates
EDTA disodium salt di-hydrate                          37.300 Sucrose                                  30000.000
Ferrous Sulphate hepta-hydrate                        27.800 Gelling Agent
Manganese Sulphate monohydrate                   16.900 Agar (Gelling Agent)              7000.000
Molybdic acid (sodium salt)                                 0.213 pH 5.75 ±0.5

 

Additional information
Weight N/A
Dimensions N/A
Size

100G, 1Kg, 500G

Directions
Directions:-
  • Suspend 41.4 gram in 800 ml distilled water (use of tap water or the potable water may form the turbid solution) with gentle steering.
  • Add heat stable ingredients before autoclaving.
  • Adjust the pH (if required) to 5.75 (with 0.1 N HCl/ NaOH) and make up the volume to 1L by adding the distilled water.
  • Boil to dissolve the medium completely including gelling agent.
  • Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C add heat labile ingredients
  • Distribute aseptically in to the desired volume in desired culture vessels in laminar air flow.

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Murashige and Skoog Medium – HX-Media

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