Lysine Lactose Broth – HX-MEDIA

Name: Lysine Lactose Broth - HX-MEDIA
SKU: HXM-LLB-01
Availability: InStock

Principle :- Lysine Lactose Broth is formulated by Falkow (1985) is also named as Falkow Lysine Broth use for detection of lysine decarboxylase by means of a color reaction in enteric bacilli. The media is composed of peptone, yeast extract, dextrose, L-lysine hydrochloride, lactose and bromocresol purple. Peptone and yeast extract provides nitrogen, vitamins, minerals, amino acids and growth factors. Dextrose and lactose are carbon or energy source for the growth of microorganisms. L-lysine hydrochloride
decarboxylated to from cadaverine.

Use :- Recommended for determination of lysine decarboxylase activity of lactose non fermenting members of Enterobacteriaceae especially Salmonellae.

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Lysine Lactose Broth – HX-MEDIA – Hexa Biotech

Directions :- Dissolved 24.00 grams in 1000 ml distilled water. Boil Lysine Lactose Broth to dissolve the medium completely. Distribute 5.00 ml into the screw capped test tubes. Sterilize by autoclaving 15 lbs pressure (121˚C) for 15 minutes. Allow to cool the tubes in an upright position and overlay with 2-3 ml of sterile mineral oil.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and
discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Performance and Evaluation :- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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