Sabouraud Dextrose Broth with Chloramphenicol – HX-Media

Sabouraud Dextrose Broth with Chloramphenicol – HX-Media

Principle:- Sabouraud dextrose broth is used for cultivation of yeasts, molds and aciduric bacteria. It is recommended by USP as a medium for microbial limit testing of pharmaceutical products and raw material used in pharmaceutical industries. The medium is prepared in accordance with the harmonized principles of USP. Medium is consisting of meat and casein peptone (1:1) and dextrose monohydrate. The meat and casein peptone provide carbonaceous, nitrogenous compounds, long chain amino acids, vitamins and other essential growth nutrients in addition to that the dextrose monohydrate serves as energy source. The high concentration of dextrose and low pH of medium favor the growth of yeasts and molds and inhibit other contaminating bacteria from pharmaceutical and clinical specimens. In addition to pH the Chloramphenicol provides excess selective for the growth of fungi and yeast.

Use:- Recommended for the cultivation of yeasts and molds from pharmaceutical products in accordance with the microbial limit testing by harmonized principles of USP.