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Azospirillum Medium w/o Agar
HomeMicro BiologyAgar Media Azospirillum Medium w/o Agar – HX-Media
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Azospirillum Medium w/o Agar – HX-Media

₹880.00 – ₹6,385.00

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Azospirillum Medium w/o Agar (Twin Pack) – HX-Media

Principle:- These mediums contain malic acid which serves as the prime carbon source and favors the growth of Azospirillum and prevent the overgrowth of other nitrogen fixers. Dipotassium phosphates help to maintain the pH of medium. Necessary trace elements like ferrous Sulphate, manganese Sulphate, sodium molybdate support growth of Azospirillum species. Divalent salts like calcium and magnesium helps metabolism of cells. Sodium chloride helps to maintain osmotic balance of cell. Due to the nitrogen fixation by diazotroph bacteria ammonia is produced, the color of the medium changed from light yellow color to greenish blue or blue (Bromothymol blue pH indicator).

Use:- Recommended for cultivation of Azospirillum species.

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SKU: N/A Categories: Agar Media, Micro Biology Tags: Agar, Agar Powder, azospirillum brasilense medium, Azospirillum Medium, azospirillum medium agar, azospirillum medium composition, azospirillum medium himedia, Azospirillum Medium w/o Agar, hexa biotech, HX-Media, nfb medium azospirillum, ScienoChem
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Description

Azospirillum Medium w/o Agar (Twin Pack) – HX-Media – Hexa Biotech

Azospirillum Medium w/o Agar Directions:-

  • Part A:- Dissolve 6.33 grams in 950 ml distilled water, boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C.
  • Part B:- Dissolve 4 grams of part B in 50 ml of sterile distilled water. Mix 950 ml of part A with 50 ml of part B in 1 liter sterile container, mix well and dispense into sterile tubes or flasks as desired.

Specimens types analyzed:- Soil samples etc.

Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life:- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

Contents
Ingredients Gram/Liter
Part A
Malic acid 5.000
Dipotassium hydrogen phosphate 0.500
Ferrous Sulphate 0.500
Manganese Sulphate 0.010
Magnesium Sulphate 0.200
Sodium Chloride 0.100
Bromothymol blue 0.002
Sodium molybdate 0.002
Calcium chloride 0.020
Part B
Potassium hydroxide 4.000
pH at 25˚C 6.8 ±0.2
Additional information
Weight N/A
Dimensions N/A
Size

100G, 1Kg, 500G

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Azospirillum Medium w/o Agar

Azospirillum Medium w/o Agar – HX-Media

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