Brain Heart Infusion Agar – HX-MEDIA

Name: Brain Heart Infusion Agar - HX-MEDIA
SKU: HXM-BHIA-01
Availability: InStock

Principle :- The medium contains protease peptone and infusions from calf brain and beef heart which serve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose is used as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium. Defibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious fungal organisms. It is a highly nutritious and can support luxuriant growth of wide variety of microorganisms. It can be further enriched by the addition of blood or rendered selective by adding different antibiotics. It is also used in the aminoglycoside and vancomycin screen test for resistant enterococci. BHA with 5-10% sheep blood and chloramphenicol (16 μg/ml) and gentamicin (5 μg/ml) will inhibit the growth of bacteria while allowing growth of dimorphic fungi.15 This agar can be used as a primary plating medium. This medium used for primary isolation of aerobic bacteria from clinical specimens. BHI with 0.5% Polysorbate 80 can be used for detecting Mycobacterium avium-intracellulare complex organisms and M. tuberculosis from blood cultures.

Use :- Recommended for cultivation of fastidious pathogenic bacteria, yeasts and molds.

Brain Heart Infusion Agar – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 52.00 grams in 1000 ml distilled. Boil Brain Heart Infusion Agar to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute in petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.

Specimens types analyzed :- Pharmaceutical samples, clinical and non-clinical samples etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

-20%