Blood Agar Base – HX-MEDIA

Name: Blood Agar Base - HX-MEDIA
SKU: HXM-BAB-02
Availability: InStock

Principle :- Blood agar base is modification of blood agar base with slight deviation in composition and pH. The media is composed of peptone, tryptone, sodium chloride and agar. Peptone and tryptone provide nitrogen, amino acids, carbon, vitamins and other necessary growth factors necessary for growth of microorganisms. Sodium chloride to maintain osmotic balance and agar as a solidifying agent. The media need supplementation of blood (5-10%), which provides additional growth factors for fastidious microorganisms and is the basis for determining hemolytic reactions. The media can also be used as general-purpose media without the addition of blood.

Use :- Recommended by APHA (2015) for isolating and cultivating fastidious microorganisms with or without added blood.

Blood Agar Base – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 40.00 grams in 1000 ml distilled water. Blood Agar Base boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C, and aseptically add 5% v/v sterile defibrinated blood. Mix well and pour into sterile petri plates. Ensure complete solidification and inoculate test sample aseptically.

Specimens types analyzed :- Pharmaceutical samples, clinical and non-clinical samples, food and dairy samples etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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