Dey-Engley Neutralizing Agar – HX-MEDIA

Principle :- Dey-Engley Neutralizing Agar is formulated as per the procedure described by Engley and Dey (1970). Media consist of Tryptone, yeast extract, dextrose, sodium thioglycollate, sodium thiosulphate, sodium bisulphite, Lecithin, polysorbate 80, Bromocresol purple and Agar. Tryptone and yeast extract provides nitrogen and carbon source, viDirections: Dissolve 54.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically in desired, allow to solidify and inoculate the test sample.tamins and other essential nutrients like vitamins and cofactors. Dextrose is an energy source or carbon source. The sodium thioglycollate, sodium thiosulphate, sodium bisulphite and lecithin, act as neutralizing substances for almost all the active products used as antiseptics and disinfectants in commercial products. Sodium thioglycollate neutralizes mercurials; sodium thiosulfate neutralizes iodine and chlorine and Sodium bisulfite neutralizes aldehydes.

Use :- Recommended for neutralizing and determining the bactericidal activity of antiseptics, disinfectants and chemical in commercial products after treatment.