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Principle :- Kings B medium is described by King et al., (1954), for non-selective isolation of pigment producing Pseudomonas Species. Media is composed of proteose peptone, dipotassium hydrogen phosphate and magnesium sulphate. Proteose peptone provide nitrogen and other necessary trace nutrients for growth of bacteria. Dipotassium phosphate and magnesium sulphate enhances pigment production. The glycerol serves as carbon source.
Use :- Recommended for the non-selective cultivation and pigment production of Pseudomonas species.
Directions :- Dissolve 23.00 grams in 1000 ml distilled water containing 15 ml glycerol. Boil Kings B Medium Broth to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
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