Ashby’s Sucrose Broth – HX-MEDIA

Name: Ashby’s Sucrose Broth - HX-MEDIA
SKU: HXM-ASSB-01
Availability: InStock

Principle :- Ashbys sucrose broth is described by Subba Rao (1977) for isolation of a non-symbiotic nitrogen fixing bacteria, Azotobacter. Ashby’s media composed of Sucrose, dipotassium phosphate, magnesium sulphate, sodium chloride, potassium sulphate, calcium carbonate. Sucrose provides carbon source and atmospheric nitrogen serves nitrogen source. Dipotassium phosphate is buffering agent. The other salts provide various essential ions required for promoting growth of Azotobacter. The presence of calcium carbonate stimulate growth and increase availability of phosphorus, sulphur and magnesium to the organisms.

Use :- Recommended for cultivation of sucrose fermenting Azotobacter from soil samples.

Ashby’s Sucrose Broth – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 25.70 grams in 1000 ml distilled water. Ashby’s Sucrose Broth sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.

Specimens types analyzed :- Soil and water samples etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and
discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Performance and Evaluation :- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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