Asparagine Broth – HX-MEDIA

Name: Asparagine Broth - HX-MEDIA
SKU: HXM-ASB-01
Availability: InStock

Principle :- Asparagine broth is an enrichment broth for Pseudomonas aeruginosa. The composition is strictly mineral base with enantiomeric forms of asparagine, magnesium sulphate, dipotassium hydrogen phosphate. DL-asparagine serve as the sole source of nitrogen. The dipotassium hydrogen phosphate act as a buffer system and magnesium sulfate perform multiple functions, magnesium ion required in a large variety of enzymatic reactions, reproduction and also acts as a buffer. Pseudomonas aeruginosa hydrolyze asparagine to aspartic acid and reproduce vigorously, the appearance of growth with or without fluorescent pigmentation is considered a presumptive test for P. aeruginosa. The microbial count can be determined by using the MPN test and conformation need to be done by subculturing on cetrimide agar.

Use :- Recommended for the cultivation of Pseudomonas aeruginosa using membrane filter technique.

Asparagine Broth – HX-MEDIA – Hexa Biotech

Directions :- Asparagine Broth dissolve 4.50 grams in 1000 ml distilled water, boil to dissolve the medium completely Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.

Specimens types analyzed :- Drinking water and packing drinks and carbonated beverages etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and
discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Performance and Evaluation :- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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