Cetrimide Agar – HX-MEDIA

Name: Cetrimide Agar - HX-MEDIA
SKU: HXM-CTRA-01
Availability: InStock

Principle :- Cetrimide agar is described by King et.al (1954) and is prepared in accordance with the harmonized principles of USP/EP/IP. Recommended as selective medium for the isolation of Pseudomonas aeruginosa in pharmaceutical testing and microbial limit testing of pharmaceutical products and raw material used in pharmaceutical industries. Media consists of pancreatic digest of gelatin, magnesium chloride, dipotassium sulfate, cetrimide and agar. The pancreatic digest of gelatin and peptone provides essential nutrients, vitamins and nitrogenous factors and growth factors required for growth of microorganisms. The magnesium chloride and potassium sulphate stimulate pyocyanin and fluorescein production. Cetrimide is the selective agent and inhibits most bacteria by acting as a detergent. The cetrimide came in contact with bacteria, causes the release of nitrogen and phosphorous from the bacterial cell other than Pseudomonas aeruginosa. Glycerol (not provided) is supplemented as a source of carbon. Agar is the solidifying agent.

Use :- Recommended for the isolation of Pseudomonas aeruginosa from pharmaceutical products in accordance with the microbial limit testing by harmonized principles of USP/EP/IP.

Cetrimide Agar – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 45.50 grams in 1000 ml distilled water containing 1% glycerol, boil Cetrimide Agar to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45°C and distribute aseptically in sterile petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and
discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Performance and Evaluation :- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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