Chapman Stone Agar – HX-MEDIA

Name: Chapman Stone Agar - HX-MEDIA
SKU: HXM-MSA-02
Availability: InStock

Principle :- Chapman stone agar is selective medium used for isolation of staphylococci from food and food products. The media is composed of tryptone, yeast extract, gelatin, lactose, mannitol, sodium chloride, dipotassium phosphate and agar. Tryptone as a source of nitrogen, vitamins and minerals. Yeast Extract provides carbon and nitrogen and B-complex vitamins which stimulate bacterial growth. Sodium Chloride, in high concentration, inhibits most bacteria other than staphylococci. Lactose and D-Mannitol are the carbohydrates. Gelatin is included for testing liquefaction. Agar is the solidifying agent.

Use :- Recommended for selective isolation of Staphylococci causing food poisoning.

Chapman Stone Agar – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 202.00 grams in 1000 ml distilled water, boil chapman stone agar to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and continuously shake while distributing in petri plates. Ensure complete solidification and inoculate test sample aseptically.
Mannitol fermentation test: Pick a colony from the medium on a cavity slide, add a drop of 0.04% brom thymol blue to the plate, and observe for the formation of a yellow color (positive reaction).
Gelatinase test: Flood the plate with 5 ml of saturated ammonium sulfate solution and incubate at 35 ± 2°C for 10 minutes. Observe for a zone of clearing around the colonies (positive reaction).

Performance and Evaluation :- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

-20%