Chapman Stone Agar – HX-MEDIA – Hexa Biotech
Directions :- Dissolve 202.00 grams in 1000 ml distilled water, boil chapman stone agar to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and continuously shake while distributing in petri plates. Ensure complete solidification and inoculate test sample aseptically.
Mannitol fermentation test: Pick a colony from the medium on a cavity slide, add a drop of 0.04% brom thymol blue to the plate, and observe for the formation of a yellow color (positive reaction).
Gelatinase test: Flood the plate with 5 ml of saturated ammonium sulfate solution and incubate at 35 ± 2°C for 10 minutes. Observe for a zone of clearing around the colonies (positive reaction).
Performance and Evaluation :- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.
Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.
Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.