Differential Reinforced Medium for Clostridia – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 29.00 grams in 1000 ml distilled water. Boil Differential Reinforced Medium for Clostridia to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in test tubes. Allow to cool and inoculate test sample aseptically. For single and double strength, add 0.5 ml filter sterilized solution, prepared by mixing equal volumes of 4% w/v solution of sodium sulphite and 7% w/v ferric citrate, to 25 ml of single strength medium or 0.4 ml and 2 ml to 10 ml and 50 ml of double strength medium respectively. Mix well

Performance and Evaluation :- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.