Letheen Broth Modified – HX-MEDIA

Name: Letheen Broth Modified - HX-MEDIA
SKU: HXM-LHMB-01
Availability: InStock

Principle :- Letheen broth modified is composed of peptone, tryptone, meat extract (equivalent of beef extract), yeast extract, sodium chloride, sodium bisulfite, lecithin and polysorbate 80. Peptone, tryptone, meat extract and yeast extract provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and trace elements for the growth of microorganisms. Sodium chloride maintains equilibrium of the medium. Sodium bisulfite neutralizes the preservative and enhances growth of microorganisms. The lecithin and tween 80 aids the recovery of bacteria from materials containing residues of disinfectant or preservatives used in cosmetics. Tween 80 and lecithin act as surface active disinfectant neutralizing agent, nullify other additives in commercial products. Lecithin also neutralizes quaternary ammonium compounds present in the cosmetics.

Use :- Recommended for screening cosmetic products for microbial contamination and evaluating the bactericidal activity of quaternary ammonium compounds.

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Letheen Broth Modified – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 42.80 grams in 1000 ml distilled water. Boil Letheen Broth Modified to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.

Specimens types analyzed :- Cosmetics and other commercial products etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and
discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Performance and Evaluation :- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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