MacConkey Agar w/ 0.15% Bile Salts, CV and NaCl – HX-Media

MacConkey Agar w/ 0.15% Bile Salts, CV and NaCl – HX-Media – Hexa Biotech

MacConkey Agar Use :- But for or the selection and subculture of E.coli in accordance with harmonized method.

Product Summary and Explanation :- MacConkey Agar have slightly selective and differential plating media mainly used for the detection and isolation of gram negative organisms from clinical, dairy, food, water, pharmaceutical and industrial sources.

It’s also recommended for the selection and recovery of the Enterobacteriaceae and related enteric gram-negative bacilli.

The medium corresponds with, was recommended by APHA can be used for the direct plating of water samples for coliform bacilli.

For the examination of food samples for food poisoning organisms had the isolation of Salmonella and Shigella species in cheese.

Lactose fermenting strains grow as red or pink and may be surrounded by a zone of acid precipitated bile.

The red colour due to production of acid from lactose, absorption of Neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8.

Lactose non-fermenting strains, such as Shigella and Salmonella colourless, transparent and typically do not alter appearance of the medium.

Storage :- On receipt, store bottles in the dark at 10 to 25° C. Avoid freezing and overheating. Do not liquefy any leftovers for a second time.

The medium may have used up to the expiration date and incubated for the recommended incubation times.

Opened bottles must be used immediately. To prepare plates or tubes from the bottled medium, it must first be liquefied.

Product Deterioration:- Do not use bottles if they show evidence of microbial contamination, discoloration, or any other signs of deterioration.

Disposal :- After use, prepared plates, specimen/sample containers and other contaminated materials must be sterilized before discarding.