Mannitol Salt Broth – HX-MEDIA

Name: Mannitol Salt Broth - HX-MEDIA
SKU: HXM-MSB-01
Availability: InStock

Principle :- Mannitol salt broth is a medium used for the detection and enumeration of Staphylococci. Mannitol salt broth is composed of pancreatic digest of casein, peptic digest of animal tissue and meat extract (equivalent to beef extract) as sources of carbon, nitrogen, vitamins and minerals. Sodium chloride, in high concentration (7.5%), act as selective agent, allow only to grow the Staphylococci and halophilic Enterobacteria and inhibit other bacteria. Phenol red is the pH indicator. D-Mannitol is the carbohydrate source. Mannitol fermentation with an accumulation of acid products, detected by the phenol red indicator which turns yellow.

Use :- Recommended for selective enrichment and detection of Staphylococci.

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Mannitol Salt Broth – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 96.00 grams in 1000 ml distilled water. Boil Mannitol Salt Broth to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C, and inoculate test sample aseptically.

Specimens’ types analyzed :- Pharmaceutical samples, clinical and non-clinical samples etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :– To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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