Rose Bengal Agar Base – HX-MEDIA – Hexa Biotech
Directions :- Dissolve 32.00 grams in 1000 ml distilled water. Boil Rose Bengal Agar Base to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
Note :- For more selectivity add 2 ml of chloramphenicol solution (0.1 g in 2ml of ethanol) in cooled agar base mix thoroughly and dispense in sterile petri plates.