Sabouraud Dextrose Agar – HX-MEDIA – Hexa Biotech
Directions :- Dissolve 65.0 grams in 1000 ml distilled water. Sterilize Sabouraud Dextrose Agar by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in to the petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.
As per IP and EP the media can be fortified with antibiotic like 0.1g of benzylpenicillin sodium and 0.1g of tetracycline per liter as sterile solution or alternatively 50 mg of chloramphenicol per liter of medium before sterilization to achieve more selectivity.
Specimens types analyzed :- Pharmaceutical samples, clinical samples, etc.
Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.