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Selenite Broth
Selenite Broth
HomeMicro BiologyAgar Media Selenite Broth (Twin Pack) – HX-Media
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Selenite Broth (Twin Pack) – HX-Media

₹725.00 – ₹6,455.00

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Selenite F Broth (Twin Pack) – HX-MEDIA

Principle :- Tryptone provides nitrogenous substances. Lactose maintains the pH of medium. Selenite is reduced by bacterial growth and alkali is produced. An increase in pH lessens the toxicity of the selenite and results in overgrowth of other bacteria. The acid produced by bacteria due to lactose fermentation serves to maintain a neutral pH.

Sodium phosphate maintains a stable pH and also lessens the toxicity of selenite.Enriched broth is subcultured on differential plating media such as Bismuth Sulphite Agar, Brilliant Green Agar, XLD Agar etc. Do not incubate the broth longer than 24 hours as inhibitory effect of selenite decreases after 6 – 12 hours of incubation.

Instruction For Use :-

  • Dissolve 4.0 grams of Part II in 1000 ml distilled water. Add 19.0 grams of Part I.
  • Mix well. Warm to dissolve the medium completely.
  • Distribute in sterile test tubes. Sterilize in a boiling water bath or free flowing steam for 10 minutes. Do not autoclave. Excessive heating is detrimental.
  • Discard the prepared medium if large amount of selenite is reduced (indicated by red precipitate at the bottom of tube/bottle).
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SKU: HXM-SLF-01 Category: Agar Media Tags: Agar, Broth, hexa biotech, Hexa Lab Analytical, HX-Media, HX-Tech, Selenite, Selenite Broth, Selenite F Broth, Twin Pack
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Description

Selenite F Broth (Twin Pack) – HX-MEDIA – Hexa Biotech

Selenite Broth Intended Use :- As enrichment media for the isolation of Salmonellae from faeces, urine or other pathological materials.

Product Summary and Explanation :- Klett first demonstrated the selective inhibitory effects of selenite and Guth used it to isolate Salmonella Typhi.

Enrichment media are routinely employed for detection of pathogens in faecal specimens as the pathogens are present in a very small number in the intestinal flora.

Hence it is useful for detecting Salmonella in the non-acute stages of illness when organisms occur in the faeces in low numbers and for epidemiological studies.

Enhance the detection of low number of organisms from asymptomatic or convalescent patients.

Packaging :- In pack size of 100 gm and 500 gm bottles.

Storage :- Dehydrated powder, hygroscopic in nature.

Besides store in a dry place, in tightly-sealed containers between 25-30°C and protect from direct sunlight.

Under optimal conditions, the medium has a shelf life of 4 years.

After the desired amount of medium has been taken out replace the cap tightly to protect from hydration.

Product Deterioration :- Do not use if they show evidence of microbial contamination, discoloration, drying or any other signs of deterioration.

Disposal :- After use, prepared plates, specimen/sample containers and other contaminated materials must be sterilized before discarding.

Additional information
Weight N/A
Size

100G, 1Kg, 500G

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Selenite Broth

Selenite Broth (Twin Pack) – HX-Media

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