Urea Agar Base – HX-MEDIA – Hexa Biotech

Urea Agar Base Intended Use :- For detection of urease producing bacteria.

Product Summary and Explanation :- Urea Agar is used to detect urease production. Urea Agar described by Christensen detected urease activity by all rapidly urease-positive.

Proteus organisms and also by other members of Enterobacteriaceae that exhibited a delayed urease reaction. This was accomplished by

• adding glucose to the medium.
• decreasing the peptone concentration and
• decreasing the buffering system, as a less buffered medium detects even smaller amount of alkali.

Prolonged incubation may cause alkaline reaction in the medium. A medium without urea serves as negative control to rule out false positive results.

Also, all urea test media rely on the alkalinity formation and so they are not specific for determining the absolute rate of urease activity.

The utilization of proteins may raise the pH to alkalinity due to protein hydrolysis and excess of amino acids liberation results in false positive reaction.

Packaging :- In pack size of 100 gm and 500 gm bottles.

Storage :- Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers between 25-30°C and protect from direct sunlight.

Under optimal conditions, the medium has a shelf life of 4 years.

When the container is opened for the first time, note the time and date on the label space provided on the container.

After the desired amount of medium has been taken out replace the cap tightly to protect from hydration.

Product Deterioration :- Do not use if they show evidence of microbial contamination, discoloration, drying or any other signs of deterioration.

Disposal :- After use, prepared plates, specimen/sample containers and other contaminated materials must be sterilized before discarding.