Triple Sugar Iron Agar – HX-MEDIA – Hexa Biotech

Triple Sugar Iron Agar Use :- But to differentiate gram-negative enteric bacilli based on carbohydrate fermentation.

Product Summary and Explanation :- TSI Agar originally proposed by Sulkin and Willett and modified by Hajna for identifying Enterobacteriaceae.

This medium complies with the recommendation of APHA, for the examination of meat and food products, for the examination of milk and dairy products.

And for microbial limit test for confirming the presence of Salmonellae and in the identification of gram-negative bacilli.

Directions :- Firstly dissolve 17.00 Grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically.

Sterilize by autoclaving at 15 lbs pressure (121 ‘C) for 15 min, kept in slight slanting position to form small slant with large butt.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product.

Must be disposed after autoclaving or incineration after completion of task.

Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample.

Must have been decontaminated and disposed of in accordance with current laboratory techniques.

Storage :- On receipt, store tubes in the dark at 2 – 8°C. So avoid freezing and overheating. Do not open until ready to use. Minimize exposure to light.

So tubed media stored as labeled until just prior to use may be inoculated but up to the expiration date and incubated for the recommended incubation times.

Product Deterioration :- But do not use tubes if they show evidence of microbial contamination, discoloration, drying or other signs of deterioration.