Simmons Citrate Agar – HX-MEDIA

Name: Simmons Citrate Agar - HX-MEDIA
SKU: HXM-SCA-01
Availability: InStock

₹750.00 – ₹6,900.00

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Simmons Citrate Agar – HX-MEDIA

Principle :- Simmons Citrate Agar is a modification of Koser’s medium (1923). Simmons citrate agar is composed of magnesium sulphate, ammonium dihydrogen phosphate, dipotassium phosphate, sodium citrate, sodium chloride, bromothymol blue and agar. Magnesium sulfate is a cofactor for variety of metabolic reactions for growth and reproduction of microorganisms. Ammonium dihydrogen phosphate is the source of nitrogen. Dipotassium Phosphate acts as a buffer. Sodium Citrate is source of carbon. Sodium Chloride maintains the osmotic balance. Bromothymol blue act as pH indicator. Agar is solidifying agent. The shift in pH turns the bromthymol blue indicator in the medium from green to blue above pH 7.6

Use :- Recommended for differentiation the members of Enterobacteriaceae on the basis of citrate utilization from clinical and non-clinical samples.

SKU: HXM-SCA-01 Categories: Agar Media, Micro Biology Tags: Agar, Broth, hexa biotech, HX-Media, HX-Tech, ScienoChem, Simmons Citrate, Simmons Citrate Agar

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Simmons Citrate Agar – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 24.28 grams in 1000 ml distilled water (the pH of distilled water must be 6.5 to 7.0). Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in test tubes. Slant the tubes to have a small butt and slant, allow to solidify. Ensure complete solidification and inoculate test sample aseptically.

Specimens types analyzed :- Pharmaceutical samples, clinical and non-clinical samples etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

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