Tryptic Soya Broth – HX-Media – Hexa Biotech
Directions:- Dissolve tryptic soya broth 30.00 grams in 1000 ml distilled water; boil to dissolve the medium completely and distribute aseptically. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
Specimens types analyzed:- Pharmaceutical samples, clinical and non-clinical samples etc.
Performance and Evaluation:- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.
Storage and Shelf Life:- Hygroscopic; keep container tightly closed. Store in cool dry place.
Disposal:- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.
Ingredients | Grams / Liter |
Tryptone | 17.000 |
Soya Peptone | 3.000 |
Sodium Chloride | 5.000 |
D-Glucose | 2.500 |
Dipotassium Hydrogen Phosphate | 2.500 |
pH (at 25 ‘C) | 7.3 ± 0.2 |






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