Vogel-Johnson Agar Medium – HX-MEDIA

Name: Vogel-Johnson Agar Medium - HX-MEDIA
SKU: HXM-VJA-01
Availability: InStock

Principle :- Vogel-Johnson agar medium (VJ medium) is composed of tryptone, yeast extract, mannitol, dipotassium phosphate, lithium chloride, glycine, phenol red and agar. Tryptone and yeast extract provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamin B complex and other growth nutrients. Mannitol is source of carbon. Dipotassium phosphate acts as buffering agent. Lithium chloride and glycine inhibits most of microorganisms except staphylococci. Phenol red is pH indicator. Agar is solidifying agent. VJ Agar selects and differentiates the coagulase positive staphylococci which ferment mannitol and reduce tellurite.

Use :- Recommended for selective isolation of mannitol fermenting Staphylococcus aureus from heavily contaminated food and clinical specimens (after addition of potassium tellurite).

Vogel-Johnson Agar Medium – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 60.00 grams in 1000 ml distilled water. Boil Vogel-Johnson Agar Medium to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C, add 20ml of tellurite solution mix well and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.

Specimens’ types analyzed :- Pharmaceutical samples, clinical and non-clinical samples etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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