Bushnell Haas Agar – HX-MEDIA

Name: Bushnell Haas Agar - HX-MEDIA
SKU: HXM-BHA-01
Availability: InStock

Principle :- Bushnell-Haas agar is modification of Bushnell Hass broth describe by Bushnell and Hass, for enrichment of hydrocarbon decomposing microorganisms. The media is composed of magnesium sulfate, calcium chloride, monopotassium phosphate, ammonium phosphate dibasic, potassium nitrate ferric chloride and agar. Magnesium sulfate, calcium chloride and ferric chloride provides necessary trace elements for growth of bacteria. Monopotassium phosphate and ammonium phosphate dibasic are buffering agents. Potassium nitrate is a
nitrogen source. The media lacks carbon source and can be fortified with hydrocarbons for evaluation of hydrocarbon decomposing ability. Agar is solidifying agent. Bushnell-Haas was recommended for the microbiological examination of fuels by the Society for Industrial Microbiology (SIM) Committee on Microbiological Deterioration of Fuels.

Use :- Recommended for isolation of hydrocarbon degrading microorganism and enumeration of fuels contaminating microorganisms.

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Bushnell Haas Agar – HX-MEDIA – Hexa Biotech

Directions for hydrocarbon decomposing ability :- Dissolve 23.27 grams in 1000 ml distilled water check. Boil Bushnell Haas Agar to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.

Specimens types analyzed :- Hydrocarbons such as kerosene, light and heavy mineral oils, paraffin wax, and gasoline etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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