Brucella Agar Base – HX-MEDIA

Name: Brucella Agar Base - HX-MEDIA
SKU: HXM-BAB-01
Availability: InStock

Principle :- Brucella agar base is composed of peptone, tryptone, yeast extract, dextrose, sodium chloride, sodium bisulphite and agar. Peptone and tryptone provides nitrogen and amino acids. Yeast extract adds essential vitamins. Dextrose is a carbon source; Sodium Bisulfite is a reducing agent and enhances growth. Sodium Chloride maintains the osmotic balance. Agar is the solidifying agent. The media can be enriched with blood (5-10%), which provides additional growth factors for fastidious microorganisms and is used to determine hemolytic reactions.

Use :- Recommended for enrichment, isolation and cultivation of Brucella or Campylobacter species from clinical and non-clinical specimens.

Brucella Agar Base – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 43.00 grams in 1000 ml distilled water. Boil Brucella Agar Base to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and distribute in petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically. Elective: To prepare Brucella Blood Agar, aseptically add 5-10% sterile defibrinated blood at 45-50°C. Mix well.

Specimens types analyzed :- Pharmaceutical samples, clinical and non-clinical samples etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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