E C Broth – HX-MEDIA

Name: E C Broth - HX-MEDIA
SKU: HXM-ECB-01
Availability: InStock

Principle :- EC broth is used for detection of coliforms during bacteriological examination of water, milk and food samples. Media was described by Hajna and Perry (1943). Media composed of tryptone, lactose, bile salt, dipotassium phosphate, potassium dihydrogen phosphate and sodium chloride. Tryptone provide nitrogen and amino acids and trace nutrients required for the growth of bacteria. Lactose is fermentable carbohydrate, allow only lactose fermenting bacteria to grow. Bile salts inhibit gram-positive bacteria including fecal streptococci. Dipotassium phosphate and potassium dihydrogen phosphate are buffering agent resist change in pH of medium. Gas production in a fermentation tube within 24 hour or less is a presumptive evidence of the presence of coliform bacteria.

Use :- Recommended for the selective enumeration of presumptive Escherichia coli by MPN method from water, food and clinical samples.

E C Broth – HX-MEDIA – Hexa Biotech

Directions: Dissolve 37.00 grams in 1000 ml distilled water. Boil E C Broth to dissolve the medium completely and distribute in test tube containing inverted Durham’s tube. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and
discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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