Phenol Red Broth Base – HX-MEDIA

Name: Phenol Red Broth Base - HX-MEDIA
SKU: HXM-PRBB-01
Availability: InStock

Principle :- Phenol red broth base is formulated by Vera (1950) is used for to study carbohydrate fermentation reaction. It is composed of proteose peptone, meat extract, sodium chloride and phenol red. Peptone and meat extract provide carbon, nitrogen sources and essential growth factors required for growth of microorganisms. The sodium chloride maintains osmatic equilibrium of the medium. Phenol red is pH indicator dye. The medium contains all the necessary nutrients required for the growth of microorganism, except carbohydrate. The desired carbohydrate is added into the medium to carbohydrate fermentation reaction. The gas production is detected by gas trapped in Durham’s tubes.

Use :- Recommended for studying carbohydrates fermentation reactions of pure cultures of microorganisms.

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Phenol Red Broth Base – HX-MEDIA – Hexa Biotech

Directions :- Dissolved 16.00 grams in 1000 ml distilled water. Boil Phenol Red Broth Base to dissolve the medium completely and distribute aseptically in test tubes containing inverted Durham’s tubes. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C. Aseptically add filter sterilized or autoclave sterilized carbohydrate solution to sterile basal medium. Mix well and inoculate test sample aseptically.

Specimens types analyzed :- Pure culture of microorganisms etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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