Bile Salt Agar – HX-MEDIA

Name: Bile Salt Agar - HX-MEDIA
SKU: HXM-BISA-01
Availability: InStock

Principle :- Bile Salt Agar is formulated as per recommended by BIS (1986) for isolation, identification and enumeration of Vibrio cholerae. The media is composed of peptone, special peptone, sodium chloride, sodium taurocholate and agar. The bile salt provides selectivity for the growth of Vibrio species, and many other gram-negative bacteria. On bile salt agar colonies of Vibrio’s have a distinctive appearance and can be easily differentiated from Escherichia coli. The medium contains peptone, special peptone (equivalence to meat extract) which provides carbonaceous, nitrogenous compounds and other essential growth nutrients. Sodium
chloride maintains osmotic equilibrium of the medium. Sodium taurocholate inhibits most of the gram-negative organisms.

Use :- Recommended by BIS committee under the specifications IS:5887 (Part V) -1976 for isolation and identification of bile tolerant bacteria responsible for food poisoning.

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Bile Salt Agar – HX-MEDIA – Hexa Biotech

Directions :- Dissolve 40.00 grams in 1000 ml distilled water. Bile Salt Agar is heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C), cool to 45-50°C. Mix well and distribute aseptically in petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.

Specimens types analyzed :- Food samples.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and
discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Performance and Evaluation :- The expected performance of the medium is liable to use as per the direction on the label when stored at optimum conditions and within expiry date.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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