Fraser Broth Base – HX-MEDIA

Name: Fraser Broth Base - HX-MEDIA
SKU: HXM-FBB-01
Availability: InStock

Principle :- Fraser Broth Base is based on the formulation of Fraser and Sperber (1988) is used for the detection of Listeria species in food products. It is composed of peptone, tryptone, yeast extract, meat extract, sodium chloride, sodium phosphate dibasic, potassium phosphate monobasic, esculin and lithium chloride. The media can be fortified with nalidixic acid and acriflavine to increase the selectivity of media. Peptone, tryptone, meat extract and yeast extract provide carbon, nitrogen sources and essential growth factors required for growth of Listeria. The high sodium chloride concentration of the medium inhibits growth of enterococci. Sodium phosphate and potassium phosphate act as buffering agents.

Use :- Recommended for selective enrichment of esculin hydrolyzing Listeria species.

Fraser Broth Base – HX-MEDIA – Hexa Biotech

Directions :- Dissolved 55.00 grams in 1000 ml distilled water. Boil Fraser Broth Base to dissolve the medium completely and distribute aseptically. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and add rehydrated contents of 1 vial Fraser selective supplement 1(RDM-FSS-01)and 2 vials of Fraser Selective supplement (RDM-FSS-02). Mix well and distribute in sterile test tube and inoculate test sample aseptically.

Specimens types analyzed :- Pharmaceutical samples, food and dairy samples etc.

Precautions to be taken :- These microbial media are intended for the in-vitro use only. All the handling, experiments, storage, and
discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines. The material should be disposed only after proper sterilization by autoclaving. Please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task.

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