OF Basal Medium – HX-MEDIA – Hexa Biotech

Product Summary and Explanation :- Hugh and Leifson developed OF Basal Medium to study oxidative and fermentative metabolism of carbohydrates by gram negative bacteria.

This criterion is used during taxonomic studies of Enterobacteriaceae.

So organisms which are not classified either as oxidative or fermentative show no change in the covered medium and an alkaline reaction in the uncovered medium.

Directions :- Firstly dissolve 9.38 grams in 1000 ml distilled water. Then sterilize OF Medium by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

And then cool to 45-50°C and add 10 ml of 10% sterile carbohydrate solution (10% Dextrose, 10% lactose or 10% Saccharose solution).

Mix well and aseptically transfer 5 ml in sterile test tube to form stab. Inoculate test sample aseptically.

Specimen’s types analyzed :- Clinical samples: Faeces or fecal and rectal screening swab samples, pure isolates etc.

Precautions to be taken :- Basically these microbial media are intended for the in-vitro use only.

All the handling, experiments, storage, and discarding should be performed with the help of skilled and knowledgeable technicians and as per the established guidelines.

The material should be disposed only after proper sterilization by autoclaving.

But please go through the MSDS of the media to avoid any accidents or in emergency.

Storage and Shelf Life :- Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers between 25-30°C and protect from direct sunlight.

Under optimal conditions, the medium has a shelf life of 4 years. Once the desired amount of medium has been taken out replace the cap tightly to protect from hydration.

Hygroscopic; keep container tightly closed. Store in cool dry place.

Disposal :- To avoid the contamination or propagation of any hazardous microbes the used.

Unusable or modified preparation of this product must be disposed after autoclaving after completion of task.