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Principle :- Pseudomonas asparagine broth is recommended by APHA for presumptive determination of Pseudomonas aeruginosa from water sources or samples. The media is composed of DL-asparagine, dipotassium phosphate and magnesium sulphate. Asparagine is the amino acid source. Dipotassium phosphate acts as buffering agent. Magnesium sulfate provides essential cations for fluorescein production.
Use :- Recommended for presumptive determination of Pseudomonas aeruginosa from water samples.
Directions :- Dissolve 4.50 grams in 1000 ml distilled water. Boil Pseudomonas Asparagine Broth to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to room temperature and inoculate test sample aseptically.
Storage and Shelf Life :- Hygroscopic; keep container tightly closed. Store in cool dry place.
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