₹750.00 – ₹5,745.00
Principle :- Yeast mannitol broth is used for cultivation of nitrogen fixing Rhizobium. It is composed of yeast extract, mannitol, dipotassium phosphate, magnesium sulphate, sodium chloride and calcium carbonate. Yeast extract serves as a good source of readily available amino acids, vitamin B complex and accessory growth factors for Rhizobia. It also aids in oxidation-reduction potential of medium in the range favorable for Rhizobia and serves as hydrogen donor in respiratory process. Magnesium sulphate and calcium carbonate provides cations essential for the growth of Rhizobia.
Use :- Recommended for cultivation of Rhizobium species.
Directions :- Dissolve 12.80 grams in 1000 ml distilled water, boil Yeast Mannitol Broth to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
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